Molecular cloning and in-depth bioinformatics analysis of type II ribosome-inactivating protein isolated from Sambucus ebulus
نویسندگان
چکیده
منابع مشابه
Ebulin 1, a nontoxic novel type 2 ribosome-inactivating protein from Sambucus ebulus L. leaves.
A novel type 2 ribosome-inactivating protein (RIP) that we named ebulin 1 has been isolated from leaves of Sambucus ebulus L. (Caprifoliaceae). In vitro ebulin 1 strongly inhibited protein synthesis by rabbit reticulocyte lysates, rat brain, and rat liver cell-free systems but did not affect in vitro plant nor bacterial protein synthesis. Ebulin 1 is composed of two subunits, a catalytic A subu...
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Ribosome-inactivating proteins (RIPs) are a group of toxic proteins that can specifically act on the universally conserved sarcin/ricin domain (S/R domain) of the largest RNA in ribosome and thus irreversibly inactivate ribosome for protein synthesis. Cinnamomin is a multifunctional type II RIP isolated in our laboratory from the mature seeds of the camphor tree. This protein has been extensive...
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OBJECTIVE Many pharmacological activities have been reported in Sambucus (S.) genus. The aim of present study was to investigate antidepressant activities of different parts of S. ebulus and S. nigra. MATERIALS AND METHODS Antidepressant activity of methanolic extracts were evaluated by forced swimming test (FST) and tail suspension tests (TST) in male Swiss albino mice. RESULTS Extracts sh...
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OBJECTIVES Giardia (G.) lamblia is a parasite that causes giardiasis in humans and other mammals. The common treatment produces unpleasant side effects. The ethnopharmacology for management of parasitic infections accelerates and guides the search for new chemical objects. This study assessed the in vitro cytotoxicity of Sambucus (S.) ebulus fruit against Cysts of G. lamblia. MATERIALS AND ME...
متن کاملCloning and Expression of Curcin, a Ribosome-Inactivating Protein from the Seeds of Jatropha curcas
Curcin, a ribosome-inactivating protein with a molecular weight of about 28.2 kD, which strongly inhibits the protein synthesis in rabbit reticulocyte lysate system with an IC50 value of about (0.19 ± 0.01) nmol/L, was purified from the seeds of Jatropha curcas L. The protein has the activity of rRNA N-glycosidase. Degenerate primers were designed based on the N-terminal partial sequence from p...
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ژورنال
عنوان ژورنال: Saudi Journal of Biological Sciences
سال: 2020
ISSN: 1319-562X
DOI: 10.1016/j.sjbs.2020.02.009